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Spaniard Carlos Sastre of the CSC team celebrates as he wins the 17th stage of the Tour de France, a 210.5-km mountain ride from Embrun to l'Alpe d'Huez July 23, 2008. REUTERS/Thierry Roge

L'ALPE D'HUEZ, France (Reuters) - Spaniard Carlos Sastre seized the Tour de France yellow jersey with victory in the last mountain stage on Wednesday but looked incapable of preventing Cadel Evans from winning the race.

Australian Evans, who was seventh in the 210.5-km stage and now trails Sastre by one minute 34 seconds overall, is expected to gain at least two minutes on the CSC rider in Saturday's decisive 53-km time trial.

Sastre attacked in the last climb of the day to Alpe d'Huez and never looked back, taking victory ahead of compatriot Samuel Sanchez, with his CSC team mate Andy Schleck of Luxembourg coming home third, both 2:03 behind.

Sastre climbed up to the ski resort in 39:31, far outside Marco Pantani's record of 37:35 in 1997.

Frank Schleck, who wore the yellow jersey at the start of the stage, could not drop Evans and now trails his team mate by 1:24.

Austrian Bernhard Kohl, who is almost sure to wear the polka-dot jersey for the best climber all the way to Paris, is third, 1:33 off the pace, with Evans fourth, one second further behind.

Silence Lotto's Evans will now set his sights on Saturday's individual time trial. He beat Sastre by 1:16 in the first stage against the clock in Cholet, though that was over 29.5 km.

"For the moment, the only thing is to recuperate so I can be fully fit for Saturday," said Sastre.

France's Remy Di Gregorio, Slovakia's Peter Velits, German Stefan Schumacher and Spaniard Ruben Perez broke away after three kilometers on Wednesday and opened up a six-minute lead.

The peloton, led by the CSC team, increased the pace and Di Gregorio and Perez were quickly swallowed. Velits broke clear as the CSC riders stepped up another gear at the foot of the Col de la Croix de Fer.

Former Giro d'Italia winner Damiano Cunego was once again left behind by the favorites' group but Russian Denis Menchov and Evans kept up the pace.

Sastre's first attack on one of the first of the 21 hairpins to Alpe d'Huez was shortlived but Evans was unable to follow the second.

"He broke away in the Alpe d'Huez and I could not follow him," said Evans.

"Normally I have an advantage in the time trial but with the yellow jersey on your back it is different. I need to have good legs on the day."

(Editing by Clare Fallon)